Optimization of fibrinase productivity from Actinomycetes

Fibrinolytic enzymes that dissolve blood clots and show promise for thrombosis therapy have been successfully identified from various sources. A wide range of microorganisms has been screened for their fibrinolytic properties. A fibrinolytic protease has been isolated from Streptomyces violaceoruber and Streptomyces spiroverticillatus culture filtrate. The purification procedure involved ammonium sulphate fractionation, dialysis, calcium phosphate gel purification and gel filtration on Sephadex G-100. By using native polyacrylamide gel electrophoresis [Native PAGE] to determine molecular weight of the enzyme. The optimum temperature for the high production of fibrinase from S. violaceoruber was 30[degree] C and from S. spiroverticillatus was 35[degree] C and the optimum pH was 9.0. The best incubation period is 6 days. The incorporation of lactose as carbon source, yeast extract as nitrogen source and MnCl[2] to culture media highly increased the production of fibrinase from the two species. The molecular weight was about 30 KDa. It exhibited fibrinolytic enzyme activity. In vitro studies revealed that fibrinase dissolves clots made by blood

MTHFR gene polymorphisms as risk factors for colorectal cancer among Egyptian patients

Polymorphisms in methylenetetrahydrofolate reductase [MTHFR], such as MTHFR C677T and A1298C, are associated with several cancers. This study aimed to evaluate the effects of MTHFR polymorphisms on colorectal cancer risk in a population from damitta Egypt. This hospital-based case-control study was conducted during 2008-2010; 64 colon cancer cases and 90 controls were enrolled. Information was collected and blood samples were obtained for assay of MTHFR C677T and A1298C polymorphisms by polymerase chain reaction-single strand conformation polymorphism [PCR-SSCP] and PCR-restriction fragment length polymorphism [PCR-RFLP] techniques. Associations between variables of interest and colorectal cancer were assessed using conditional logistic regression. Increased risk of colorectal cancer was associated with the MTHFR C677 TT genotype of C677T polymorphism [OR [adj] = 24.0; 95% CI: 1.34-429.1; P value for interaction = 0.001]. The 1298AC genotype and C allele was associated with a statistically significant lower risk among subjects [OR, 3.85; 95% CI, 1.78-8.33; P value for interaction=.0005 and OR, 1.88; 95% CI, 1.16-3.059 P value for interaction=0.01], respectively. MTHFR 1298 AA genotype and A allele was found to be associated with a significantly decreased risk for colorectal cancer [OR = 0.25, 95% CI 0.11-0.52; P value for interaction= 0.0005 and OR = 0.52, 95% CI 0.32-0.85 P value for interaction= 0.01and], respectively. There was no clear relation between colorectal adenomas and those with the 1298 CC genotype. The combined CC, AA [corrected] genotypes and the CT+AA [corrected] genotypes and the TT+ AC were associated with a statistically significant lower risk for developing colorectal cancer [P value for interaction= 0.03, 0.02, 0.001], respectively. The findings suggest an interaction between the MTHFR genotype and colorectal adenomas among Egyptian patients